Multimodal imaging to decode immunological landscapes
The Department of Immunodynamics investigates immunological mechanisms that underlie diseases such as persistent infections and cancer. We employ state of the art approaches, such as imaging mass spectrometry, bioinformatics and microscopy to understand the mechanisms that spatially regulate leukocyte migration and function. We develop interdisciplinary co-registration algorithms that combine multimodal imaging approaches, such as immunohistochemistry (IHC), matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) and liquid chromatography with tandem mass spectrometry (LC-MS/MS). Our overall aim is to decode immunological landscapes to develop novel therapeutic approaches to treat diseases.
Crosstalk of tissue macrophages and neutrophils
Neutrophils are continuously generated within the bone marrow and released into the circulation. They rapidly migrate into infected tissues to not only significantly contribute to inflammation, but also to the antimicrobial process. However, the mechanisms that regulate the interstitial migration and the function of neutrophils in infected organs are incompletely understood. We use state of the imaging techniques to analyse the role of tissue macrophages, which form a dense network of sentinel cells within tissues, for the regulation of neutrophil migration and function in inflamed tissues.
Macrophages
Resident and inflammatory macrophages
Regulation of Immunology
Induction of immunity
Regulation of Immunology
Induction of immunity
Neutrophils
Recruitment from the circulation
Interstitial migration
Crossing of barriers in Immunology
Interstitial migration
Crossing of barriers in Immunology
Immunology
Cutting edge microscopy
Computational analysis in Immunology Proteomics and MALDI Imaging
Computational analysis in Immunology Proteomics and MALDI Imaging